Soluble expression and purification of synthetic human bone morphogenetic protein-2 in Escherichia coli.
BMB Rep
; 41(5): 404-7, 2008 May 31.
Article
em En
| MEDLINE
| ID: mdl-18510873
ABSTRACT
A 345-bp gene that encodes human bone morphogenetic protein-2 (hBMP-2) has been synthesized. The codon usage of the resulting gene was modified to include those triplets that are utilized in highly expressed Escherichia coli genes. The hBMP-2 gene was efficiently expressed in E. coli as a soluble and active protein. Since the recombinant hBMP-2 was readily solublized, no further solublization steps were required throughout purification. No additional tagging residues were introduced into the synthetic hBMP-2 gene product. The developed synthetic gene is a promising approach for scaling-up the soluble expression of hBMP-2.
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Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta
/
Proteínas Morfogenéticas Ósseas
/
Escherichia coli
/
Genes Sintéticos
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article