LC-MS for protein characterization: current capabilities and future trends.
Expert Rev Proteomics
; 5(3): 435-44, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18532911
ABSTRACT
With advances in ionization methods and instrumentation, liquid chromatography (LC)/mass spectrometry (MS) has become a powerful technology for protein characterization. This review article will describe the general approaches on LC-MS analysis in protein characterization, including bottom-up and top-down strategies. Discussions will be given on characterization of recombinant proteins, and post-translational and protein modifications such as disulfide bonds, glycosylation and phosphorylation using LC-MS. New research directions in this area will also be presented to illustrate future prospects of LC-MS in protein characterization, including application to proteomics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Proteínas
/
Cromatografia Líquida
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article