[Research on the application of supercritical fluid extraction of diosgenin of Smilax china].

ABSTRACT

OBJECTIVE: To Study the optimum extraction conditions of diosgenin by CO2 Supercritical Fluid Extraction ( SFE). METHODS: Uniform design was used to select the technology of CO2 SFE. The pressure, temperature and modifer concentration were discussed to try to find out the best condition. Samples were analyzed with a reverse phase HPLC system to calculate the yield. Data was processed by SPSS statistical analysis software. RESULTS: The best extraction condition in SFE as follows extraction pressure 25 MPa, temperature 67 degrees C, seperation pressure 10 MPa, modifer concentration 95%. CONCLUSION: CO2 SFE method acquires more yield than that of traditional organic solvent extraction method in pharmacopoeia, providing scientific support on improving the extraction method of diosgenin.