Impact of maternal and neonatal factors on CD34+ cell count, total nucleated cells, and volume of cord blood.

ABSTRACT

The engraftment outcome of UCB transplantation is highly dependent on cell number. It would be useful to predict CB cell content using information of donor-related variables before cell processing. In this study, CBs were obtained from 1312 single-birth term deliveries in the Buddhist Tzu Chi Stem Cells Center from January 2001 to June 2006. We evaluated whether maternal factors, such as age and race, have an effect on laboratory parameters of hematopoietic content, including CD34+ cell counts, TNCs, and cord blood volume. We also studied the impact of neonatal factors, such as delivery method, gestational age, sex, birth weight, and birth order on the same parameters. In multivariate analysis, babies delivered via Cesarean section had more CD34+ cells and volume, but lower TNCs. Similar results were found for either babies of shorter gestational age or in male infants. Babies with larger birth weight had higher CD34+ cell volume, and TNC, while mothers with fewer previous live births had CB with more TNCs. Maternal age and race had no effect on these laboratory parameters. To conclude, our results suggest that neonatal factors affect CB cell yields. TNCs tend to be more affected by different variables than CD34+ cell counts and volume. These findings may help in collecting CB efficiently and improve the CB transplantation rate.