Epicatechin protects auditory cells against cisplatin-induced death.
Apoptosis
; 13(9): 1184-94, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18670884
ABSTRACT
Cisplatin, a chemotherapeutic drug that is widely used to treat various cancers, promotes ototoxicity at higher doses. In this study, the effect of epicatechin (EC) on cisplatin-induced hair cell death was investigated in a cochlear organ of Corti-derived cell line, HEI-OC1, and in vivo in zebrafish. Cisplatin promoted apoptosis and altered mitochondrial membrane potential (MMP) in HEI-OC1 cells. EC inhibited cisplatin-induced apoptosis and intracellular reactive oxygen species (ROS) generation. Labeling of zebrafish lateral line hair cells by the fluorescent dye YO-PRO1 was lost upon exposure to cisplatin, and EC protected against this cisplatin-induced loss of labeling in a dose-dependent manner. Scanning and transmission electron micrographs showed that treatment with EC protected against cisplatin-induced loss of kinocilium and stereocilia in zebrafish neuromasts. These results suggest that EC prevents cisplatin-induced ototoxicity by blocking ROS generation and by preventing changes in MMP.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Catequina
/
Cisplatino
/
Citoproteção
/
Células Ciliadas Auditivas
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article