Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.
Biochim Biophys Acta
; 1777(10): 1378-83, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18692019
ABSTRACT
Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Mitocondriais
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Linhagem Celular Tumoral
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Insulinoma
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Canais Iônicos
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article