Syx, a RhoA guanine exchange factor, is essential for angiogenesis in Vivo.
Circ Res
; 103(7): 710-6, 2008 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-18757825
ABSTRACT
Rho GTPases play an important and versatile role in several biological processes. In this study, we identified the zebrafish ortholog of the mammalian Rho A guanine exchange factor, synectin-binding guanine exchange factor (Syx), and determined its in vivo function in the zebrafish and the mouse. We found that Syx is expressed specifically in the vasculature of these organisms. Loss-of-function studies in the zebrafish and mouse point to a specific role for Syx in angiogenic sprouting in the developing vascular bed. Importantly, vasculogenesis and angioblast differentiation steps were unaffected in syx knockdown zebrafish embryos, and the vascular sprouting defects were partially rescued by the mouse ortholog. Syx knockdown in vitro impairs vascular endothelial growth factor-A-induced endothelial cell migration and angiogenesis. We have also uncovered a potential mechanism of endothelial sprout guidance in which angiomotin, a component of endothelial cell junctions, plays an additive role with Syx in directing endothelial sprouts. These results identify Syx as an essential contributor to angiogenesis in vivo.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Neovascularização Fisiológica
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Proteína rhoA de Ligação ao GTP
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Fatores de Troca do Nucleotídeo Guanina
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Proteínas de Peixe-Zebra
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article