Misacylation of pyrrolysine tRNA in vitro and in vivo.
FEBS Lett
; 582(23-24): 3353-8, 2008 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-18775710
ABSTRACT
Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacterial-type seryl-tRNA synthetase in vitro and in vivo in Escherichia coli. Compared to the M. barkeri Fusaro tRNA, the M. barkeri MS tRNAPyl contains two base changes; a G3U70 pair, the known identity element for E. coli alanyl-tRNA synthetase (AlaRS). While M. barkeri MS tRNAPyl cannot be alanylated by E. coli AlaRS, mutation of the MS tRNAPyl A4U69 pair into C4G69 allows aminoacylation by E. coli AlaRS both in vitro and in vivo.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência de Lisina
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Methanosarcina barkeri
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RNA Arqueal
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Aminoacilação de RNA de Transferência
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Lisina
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article