Isolation and spectral characterization of Photosystem II reaction center from Synechocystis sp. PCC 6803.
Photosynth Res
; 98(1-3): 293-302, 2008.
Article
em En
| MEDLINE
| ID: mdl-18780157
ABSTRACT
We isolated highly-purified photochemically active photosystem (PS) II reaction center (RC) complexes from the cyanobacterium Synechocystis sp. PCC 6803 using a histidine-tag introduced to the 47 kDa chlorophyll protein, and characterized their spectroscopic properties. Purification was carried out in a one-step procedure after isolation of PS II core complex. The RC complexes consist of five polypeptides, the same as in spinach. The pigment contents per two molecules of pheophytin a were 5.8 +/- 0.3 chlorophyll (Chl) a and 1.8 +/- 0.1 beta-carotene; one cytochrome b(559) was found per 6.0 Chl a molecules. Overall absorption and fluorescence properties were very similar to those of spinach PS II RCs; our preparation retains the best properties so far isolated from cyanobacteria. However, a clear band-shift of pheophytin a and beta-carotene was observed. Reasons for these differences, and RC composition, are discussed on the basis of the three-dimensional structure of complexes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexo de Proteína do Fotossistema II
/
Synechocystis
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article