Protective immunity against challenge with Leishmania (Leishmania) chagasi in beagle dogs vaccinated with recombinant A2 protein.
Vaccine
; 26(46): 5888-95, 2008 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-18786587
ABSTRACT
In this study, we investigated in dogs the immunogenicity and protective immunity against Leishmania (Leishmania) chagasi infection induced by vaccination with a formulation containing the recombinant A2 protein, an amastigote specific antigen, and saponin. Vaccinated animals produced significantly increased levels of total IgG and IgG2, but not IgG1 anti-A2 antibodies, and remained negative in conventional leishmaniasis serodiagnostic methods. Significantly increased IFN-gamma and low IL-10 levels were detected in vaccinated animals before and after challenge, as compared to control animals. Importantly, while the symptoms onset appeared as early as three months after infection in most control dogs, 14 months after challenge, 5 out of 7 vaccinated dogs remained asymptomatic. Therefore, immunization with rA2 antigen was immunogenic and induced partial protection in dogs, and allowed the serological differentiation between vaccinated and infected animals, an important requirement for a canine visceral leishmaniasis (CVL) vaccine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Protozoários
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Vacinas Protozoárias
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Leishmania infantum
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Doenças do Cão
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Leishmaniose Visceral
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Antígenos de Protozoários
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article