[Isolation and fermentation condition of milk-clotting enzyme producing strain from glutinous rice wine].
Sheng Wu Gong Cheng Xue Bao
; 24(6): 999-1003, 2008 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-18807982
ABSTRACT
Glutinous rice wine is a traditional food in south of China and it can coagulate milk. It has been proved that its function of coagulating milk is because of the presence of milk-clotting enzyme produced by microorganisms in glutinous rice wine. The aim of this work is to isolate milk-clotting enzyme producing strain from glutinous rice wine and study the fermentation condition. We screened out four bacteria and fungus by gradient dilution. It was proved that mold played the most important role in the production of milk-clotting enzyme. This is further confirmed by casein plate method. The optimization of fermentation conditions revealed that two times concentrated potato medium supplemented with 5% glucose without additional nitrogen was better for production of the enzyme. The enzyme activity was increased 144% under the conditions established.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Vinho
/
Leveduras
/
Ácido Aspártico Endopeptidases
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article