Genotyping of hepatitis B virus--genotypes a to g by multiplex polymerase chain reaction.
Intervirology
; 51(4): 247-52, 2008.
Article
em En
| MEDLINE
| ID: mdl-18812698
ABSTRACT
OBJECTIVES:
Eight genotypes (A-H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples.METHODS:
To establish a simple and accurate genotyping method, the study collected 369 HBV complete genomic sequences from the GenBank database. Type-specific primers were also designed that separated HBV genotypes A to G by multiplex polymerase chain reaction.RESULTS:
By comparison with the traditional restriction fragment length polymorphism method, over 93% of 441 samples were accurately genotyped by current assay, with a higher detection rate and sensitivity to detect mixed HBV infections.CONCLUSIONS:
This methodology can be applied only to areas prevalent with HBV genotypes A to G. However, it provides an efficient alternative for clinical diagnosis and large-scale studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Hepatite B
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article