Dirofilaria immitis and Wolbachia-derived antigens: its effect on endothelial mammal cells.
Vet Parasitol
; 158(3): 223-31, 2008 Dec 10.
Article
em En
| MEDLINE
| ID: mdl-18922634
ABSTRACT
Antigens of both Dirofilaria immitis and Wolbachia symbiont bacteria are implicated in the inflammatory pathology of heartworm infection. The aim of the present study was to compare the stimulatory capacity of in vitro cultures of vascular endothelial cells by the adult somatic antigens of D. immitis (DiSA) and the recombinant form of the Wolbachia surface protein (rWSP), during the first 24h of stimulation. Our results indicate a different stimulatory activity of the two antigens. Both the DiSA and rWSP stimulate the production of the enzymes responsible of the arachidonic acid metabolism, cyclooxygenase-2, 5-lipoxygenase (5-LO), and leukotriene B4. Only DiSA stimulates the production of prostaglandin E2. Related to the adhesion molecules, the DiSA stimulates the expression of intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1), whereas rWSP stimulates ICAM-1, PECAM-1, and vascular cell adhesion molecule-1 (VCAM-1). Expression of E-cadherin and vascular endothelial growth factor also were stimulated by rWSP. Neither of the two antigens altered the basic physiological mechanisms of endothelial cells, such as cell proliferation, cell cycle, or apoptosis. The biological and pathological significance of these finding are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
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Wolbachia
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Dirofilaria immitis
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Proteínas de Membrana
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Antígenos de Helmintos
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article