Real-time PCR in a plastic chip based on solid state FRET.
Langmuir
; 24(23): 13266-9, 2008 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-18980361
ABSTRACT
We show a novel solid state optical detection platform, integrated in a plastic biochip, based on colloidal nanocrystal FRET donors. The approach exploits a "smart" polymeric layer with both optical and biorecognition properties that allows real-time monitoring of biomolecular interactions and quantitative analyses of real-time PCR. The proposed strategy, demonstrated here for DNA detection, may open interesting perspectives for a wide range of applications, such as for proteomic studies.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Transferência Ressonante de Energia de Fluorescência
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article