Characterisation of Interleukin-8 and monocyte chemoattractant protein-1 expression within the abdominal aortic aneurysm and their association with mural inflammation.
Eur J Vasc Endovasc Surg
; 37(1): 46-55, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-19008128
ABSTRACT
OBJECTIVES:
Abdominal aortic aneurysms (AAAs) are characterised by chronic transmural inflammation. This study investigated the expression of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) within the AAA, and their relationship with mural inflammation.METHODS:
Biopsies were obtained from 25 AAAs, 15 abdominal aortas, and 10 atherosclerotic thoracic aortas. IL-8 and MCP-1 expression was measured in homogenised specimens by ELISA. Infiltrate composition and localised expression of IL-8 and MCP-1 were determined through immunohistochemistry.RESULTS:
ELISA analysis demonstrated that IL-8 and MCP-1 were raised in the AAA compared to the controls [(IL-8, AAA vs. abdominal aorta >28-fold, P<.001; AAA vs. thoracic aorta >28-fold, P<.001) (MCP-1, AAA vs. abdominal aorta 9-fold, P<.001; AAA vs. thoracic aorta 19-fold, P<.001)]. Immunohistochemistry revealed that IL-8 was localised to the inflammatory infiltrate, which consisted predominantly of CD3(+) T- and CD20(+) B-lymphocytes. MCP-1 was predominantly expressed by CD68(+) macrophages. Increasing IL-8 expression was associated with an increase in mural inflammation, and an increase in CD3(+) T-lymphocytes of CD4(+) phenotype within the infiltrate population.CONCLUSION:
Pathways involving IL-8 and MCP-1 may be involved in AAA pathogenesis. IL-8 may be directly involved in the chemotaxis of T(H)-lymphocytes into the AAA wall.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Interleucina-8
/
Aneurisma da Aorta Abdominal
/
Quimiocina CCL2
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article