Phosphorylation of nucleosides and nucleotides with inorganic monoimido-cyclo-triphosphate.

ABSTRACT

The phosphorylation of nucleosides (adenosine, guanosine, cytidine, and uridine) and nucleotides (adenosine 5'-monophosphate, guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate) has been achieved using inorganic monoimido-cyclo-triphosphate (MCTP, Na(3)P(3)O(8)NH) in aqueous solution. In this reaction, the 2'-OH or 3'-OH group of the beta-D-ribofuranose unit was phosphorylated and the total yield was more than 30% and 14%, respectively. The main products were 2'-diphosphoramidophosphononucleoside and 2'-diphosphoramidophosphononucleoside 5'-monophosphate.