Significance and expression of atypical protein kinase C-iota in human hepatocellular carcinoma.
J Surg Res
; 154(1): 143-9, 2009 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-19101699
ABSTRACT
BACKGROUND AND OBJECTIVE:
Previous studies have indicated that abnormal expression of atypical protein kinase C (aPKC-iota) plays a critical role in occurrence and progression of malignant tumor. This study analyzed the correlation of aPKC-iota with clinicopathology in hepatocarcinoma and Cyclin E and investigated molecular mechanisms of invasion and metastasis of hepatocellular carcinoma. MATERIALS ANDMETHODS:
The expression of the aPKC-iota gene was examined by reverse transcription-polymerase chain reaction in 7 specimens of normal liver tissues and 43 of hepatoma and adjacent tissues. Expression of aPKC-iota and Cyclin E protein was detected using immunohistochemistry and Western blot. Finally, we analyzed the correlation of aPKC-iota with clinicopathologic characteristics and invasion of hepatoma.RESULTS:
The expression value (0.844 +/- 0.315) of aPKC-iota gene is obviously higher in hepatoma than the value (0.530 +/- 0.217) in adjacent tissues and the value (0.372 +/- 0.130) in normal tissue (P = 0.009). The positive expression rate (58.1%) of aPKC-iota protein in hepatoma is remarkably higher than the rate (23.3%) of adjacent tissues. The expression of aPKC-iota has a positive correlation with the expression of Cyclin E, differentiation degree, and invasion of tumor (P < 0.05).CONCLUSIONS:
Differentiation degree and invasion of hepatoma are related to the expression of aPKC-iota, which plays an important role in invasion and metastasis of hepatoma.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
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Carcinoma Hepatocelular
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Isoenzimas
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Neoplasias Hepáticas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article