Growth and adipogenic differentiation of mesenchymal stromal bone marrow cells during culturing in 3D macroporous agarose cryogel sponges.
Bull Exp Biol Med
; 146(1): 129-32, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-19145370
ABSTRACT
We studied the possibility of population of macroporous agarose cryogel sponges by mesenchymal stromal bone marrow cells with their subsequent adipogenic differentiation. After 7-day culturing of mesenchymal stromal cells in agarose cryogel, the level of cell proliferation was 35%. After 3-week culturing in a medium inducing adipogenesis we observed accumulation of intracellular neutral lipids positively stained with Oil Red O. These findings can be used for the development of bioengineering constructions of the adipose tissue on the basis of spongy carriers.
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Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Proteínas Sanguíneas
/
Fibronectinas
/
Técnicas de Cultura de Células
/
Adipogenia
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article