GM-CSF accelerates proliferation of endothelial progenitor cells from murine bone marrow mononuclear cells in vitro.
Cytokine
; 45(3): 174-8, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19147372
ABSTRACT
OBJECTIVE:
To test whether the GM-CSF accelerates the proliferation of bone marrow endothelial progenitor cells (BM EPCs).METHODS:
BM EPCs were induced by endothelial cell conditioned medium (EC-CM). The effect of different concentrations of GM-CSF on the proliferation of BM EPCs was evaluated by the formation of EC-cols, MTT assay, and cell cycle assay. The single progenitor cell growth curves were quantified.RESULTS:
The data indicated that GM-CSF accelerated the proliferation of BM EPCs both in colony numbers and colony size. MTT confirmed the effect of GM-CSF on accelerating the proliferation of BM EPCs. The single colony experiments showed that EC-cols expressed different proliferation capacity, suggesting that the EC-cols with different proliferation potentials might have been derived from different levels of immature progenitors. The cell cycle assay showed that the rate of cells entering into S phase was 9.3% in the group treated with GM-CSF and 2.1% in the controls. Furthermore, these cells displayed the specific endothelial cell markers and formed capillary-like structures.CONCLUSIONS:
GM-CSF accelerates proliferation of BM EPCs. The potential beneficial of GM-CSF in the application of treating vascular ischemic patients is promising.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Células Endoteliais
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article