Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: implications for the biological properties of AML blasts.
Leuk Res
; 33(10): 1409-16, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19181379
ABSTRACT
FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with internal tandem duplications (ITDs) accounting for up to 30% of its mutations. To analyze the impact of individual ITDs on the expression profile of immature myeloid cells, we have established 32D cell lines expressing nine different FLT3/ITDs isolated from AML patients and subjected them to whole genome expression profiling and 2DE/LC/MS proteomics. Our data indicate that in comparison to the controls, FLT3/ITD-positive 32D cells exhibit less mature expression profiles resembling early hematopoietic progenitors. Moreover, our results suggest that there exist biological differences among individual ITD variants.
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Base de dados:
MEDLINE
Assunto principal:
Variação Genética
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Leucemia Mieloide Aguda
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Crise Blástica
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Sequências de Repetição em Tandem
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Tirosina Quinase 3 Semelhante a fms
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article