Chapter 2. Cell-cycle regulation of histone mRNA degradation in Mammalian cells: role of translation and oligouridylation.

ABSTRACT

Replication-dependent histone mRNAs are coordinately regulated in parallel with DNA replication. Histone mRNAs accumulate to high levels only in S-phase cells and are degraded rapidly at the end of S phase or when DNA replication is inhibited in S-phase cells. The unique 3' end on histone mRNAs is the cis element responsible for the regulation of histone mRNA degradation. This chapter describes the approaches used to demonstrate the connection between translation of histone mRNA and its degradation as well as the pathway of histone mRNA degradation in mammalian cells. In particular, the initial step in histone mRNA degradation is attachment of an oligo(U) tail to the 3' end of histone mRNA, providing a platform for binding factors that trigger mRNA degradation.