Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells.
J Zhejiang Univ Sci B
; 10(2): 93-102, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-19235267
ABSTRACT
OBJECTIVE:
To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells.METHODS:
HT-29 cells were treated with curcumin (0 approximately 80 micromol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR).RESULTS:
Curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10 approximately 80 micromol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner.CONCLUSION:
These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Curcumina
/
Mitocôndrias
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Antineoplásicos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article