Liquid chromatography-tandem mass spectrometry for the determination of methylprednisolone in rat plasma and liver after intravenous administration of its liver-targeted dextran prodrug.
J Chromatogr B Analyt Technol Biomed Life Sci
; 877(10): 927-32, 2009 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-19269263
ABSTRACT
A specific and sensitive liquid chromatography (LC)-tandem mass spectrometric method for quantitative determination of methylprednisolone (MP) in rat plasma and liver was developed and validated using triamcinolone acetonide as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma and liver. The separation of MP was performed on a C(18) column with a mobile phase of acetonitrile0.5% formic acid aqueous solution (8515, v/v) over 4min. The assay was based on the selected reaction monitoring transitions at m/z 375-->161 for MP in plasma, 375-->357 for MP in liver, and 435-->415 for internal standard in both plasma and liver. The lower limit of quantification was 20ng/mL based on 100microL of plasma or liver homogenate. Intra- and inter-day assay variations were
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Base de dados:
MEDLINE
Assunto principal:
Metilprednisolona
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Pró-Fármacos
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Cromatografia Líquida
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Dextranos
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Espectrometria de Massas em Tandem
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Fígado
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article