Bilateral periprosthetic joint infection caused by Salmonella enterica serotype Enteritidis, and identification of Salmonella sp using molecular techniques.
Int J Infect Dis
; 13(6): e463-6, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19269872
ABSTRACT
Salmonella septic arthritis is rare. Our objective was to identify bacterial species from joint fluid using a broad-range real-time PCR and pyrosequencing technique. We describe a case of bilateral Salmonella enterica serotype Enteritidis infection of right and left total knee arthroplasties. DNA was extracted from the joint fluid of the left knee, amplified by PCR, and the amplicons were evaluated by pyrosequencing. The patient was treated with ciprofloxacin, and the polyethylene liners were replaced in both knees. The results of pyrosequencing detected a Salmonella species. To the best of our knowledge, this is the first report describing the detection of Salmonella in joint fluid by universal PCR followed by pyrosequencing.
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Base de dados:
MEDLINE
Assunto principal:
Salmonella enteritidis
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Infecções por Salmonella
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Reação em Cadeia da Polimerase
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Infecções Relacionadas à Prótese
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Análise de Sequência de DNA
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Artroplastia do Joelho
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article