Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer.
Front Biosci (Landmark Ed)
; 14(10): 3634-40, 2009 01 01.
Article
em En
| MEDLINE
| ID: mdl-19273299
ABSTRACT
Riboflavin carrier protein (RCP) is a growth- and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was over-expressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.
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1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Neoplasias da Próstata
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article