The effect of obesity on intrahepatic cytokine and chemokine expression in chronic hepatitis C infection.
Gut
; 59(3): 397-404, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-19289381
ABSTRACT
BACKGROUND:
Obese subjects with chronic hepatitis C virus (HCV) infection have more rapidly progressive liver disease. Objective In this study, we aimed to compare the hepatic cytokine and chemokine profiles in obese and lean subjects with chronic HCV infection using qRT-PCR and immunohistochemistry.METHODS:
Liver biopsies from 55 subjects were studied, including 20 with chronic hepatitis C, 25 with non-alcoholic fatty liver disease and 10 subjects with non-diseased liver.RESULTS:
Compared to the control groups, the liver injury in chronic hepatitis C was characterised by increased expression of several T-helper-1 cytokines including interferon-gamma and tumour necrosis factor-alpha, and chemokines such as RANTES, IP-10 and MCP-1. In particular, in comparison with lean (BMICONCLUSIONS:
The data provide important mechanistic information on the cause of hepatic injury in obese-HCV subjects including (1) enhanced T helper-1 cytokine response patterns-to promote hepatocellular injury; (2) increased expression of the chemokines IP-10 and MCP-1 at both the mRNA and protein levels-to enhance inflammatory cell recruitment; (3) differing localisation of these chemokines within the liver of obese-HCV versus lean-HCV subjects-implying different inducing stimuli and; (4) increased CD3 expression in the liver of obese-HCV subjects-concordant with the increased expression of T cell chemoattractants.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citocinas
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Hepatite C Crônica
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Fígado
/
Obesidade
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article