Focal adhesion kinase modulates cell adhesion strengthening via integrin activation.
Mol Biol Cell
; 20(9): 2508-19, 2009 May.
Article
em En
| MEDLINE
| ID: mdl-19297531
ABSTRACT
Focal adhesion kinase (FAK) is an essential nonreceptor tyrosine kinase regulating cell migration, adhesive signaling, and mechanosensing. Using FAK-null cells expressing FAK under an inducible promoter, we demonstrate that FAK regulates the time-dependent generation of adhesive forces. During the early stages of adhesion, FAK expression in FAK-null cells enhances integrin activation to promote integrin binding and, hence, the adhesion strengthening rate. Importantly, FAK expression regulated integrin activation, and talin was required for the FAK-dependent effects. A role for FAK in integrin activation was confirmed in human fibroblasts with knocked-down FAK expression. The FAK autophosphorylation Y397 site was required for the enhancements in adhesion strengthening and integrin-binding responses. This work demonstrates a novel role for FAK in integrin activation and the time-dependent generation of cell-ECM forces.
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Base de dados:
MEDLINE
Assunto principal:
Integrinas
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Integrina alfa5beta1
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Proteína-Tirosina Quinases de Adesão Focal
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Fibroblastos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article