Single molecule-sensitive probes for imaging RNA in live cells.
Nat Methods
; 6(5): 347-9, 2009 May.
Article
em En
| MEDLINE
| ID: mdl-19349979
ABSTRACT
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA
/
Técnicas de Sonda Molecular
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article