Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade.
Biochem Biophys Res Commun
; 381(1): 75-80, 2009 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-19351598
ABSTRACT
Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBPalpha and PPARgamma but not of C/EBPbeta. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPbeta induces PPARgamma and C/EBPalpha expressions, by controlling the MAPK pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adipócitos
/
Sistema de Sinalização das MAP Quinases
/
Adipogenia
/
Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article