Comprehensive identification of cytochrome p450 isoforms from solubility-based fractionated rat liver microsomes.
Drug Metab Lett
; 1(4): 281-6, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-19356056
ABSTRACT
Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.
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Base de dados:
MEDLINE
Assunto principal:
Microssomos Hepáticos
/
Cromatografia Líquida
/
Sistema Enzimático do Citocromo P-450
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article