In vitro selection of allosteric ribozymes.
Methods Mol Biol
; 535: 45-57, 2009.
Article
em En
| MEDLINE
| ID: mdl-19377981
ABSTRACT
In vitro selection techniques offer powerful and versatile methods to isolate nucleic acid sequences with specific activities from huge libraries. The present protocol describes an in vitro selection strategy for the de novo selection of allosteric self-cleaving ribozymes responding to virtually any drug of choice. We applied this method to select hammerhead ribozymes inhibited specifically by doxycycline or pefloxacin in the sub-micromolar range. The selected ribozymes can be converted into classical aptamers via insertion of a point mutation in the catalytic center of the ribozyme.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pefloxacina
/
RNA Catalítico
/
Doxiciclina
/
Técnica de Seleção de Aptâmeros
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article