Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells.
Inflamm Res
; 58(10): 705-15, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19390785
ABSTRACT
OBJECTIVE AND DESIGN:
We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells.METHODS:
The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry.RESULTS:
Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells.CONCLUSION:
These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quercetina
/
Degranulação Celular
/
Heme Oxigenase-1
/
Hipersensibilidade
/
Mastócitos
/
Antioxidantes
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article