High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells.
World J Gastroenterol
; 15(17): 2089-96, 2009 May 07.
Article
em En
| MEDLINE
| ID: mdl-19418581
ABSTRACT
AIM:
To analyze the relevance of the microRNA miR-196a for colorectal oncogenesis.METHODS:
The impact of miR-196a on the restriction targets HoxA7, HoxB8, HoxC8 and HoxD8 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) after transient transfection of SW480 cancer cells. The miR-196a transcription profile in colorectal cancer samples, mucosa samples and diverse cancer cell lines was quantified by RT-PCR. Transiently miR-196a-transfected colorectal cancer cells were used for diverse functional assays in vitro and for a xenograft lung metastasis model in vivo.RESULTS:
HoxA7, HoxB8, HoxC8 and HoxD8 were restricted by miR-196a in a dose-dependent and gene-specific manner. High levels of miR-196a activated the AKT signaling pathway as indicated by increased phosphorylation of AKT. In addition, high levels of miR-196a promoted cancer cell detachment, migration, invasion and chemosensitivity towards platin derivatives but did not impact on proliferation or apoptosis. Furthermore, miR-196a increased the development of lung metastases in mice after tail vein injection.CONCLUSION:
miR-196a exerts a pro-oncogenic influence in colorectal cancer.
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Base de dados:
MEDLINE
Assunto principal:
Fenótipo
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Neoplasias Colorretais
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MicroRNAs
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article