Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase.
Virology
; 389(1-2): 75-81, 2009 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-19427010
ABSTRACT
The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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Replicação Viral
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Proteínas Serina-Treonina Quinases
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Herpesvirus Humano 4
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Infecções por Vírus Epstein-Barr
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article