Effect of triterpene saponins from roots of Ampelozizyphus amazonicus Ducke on diuresis in rats.
J Ethnopharmacol
; 123(2): 275-9, 2009 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-19429372
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Ampelozizyphus amazonicus Ducke is a plant used in Brazilian folk medicine to both prevent malaria and act as a depurative. AIM OF THE STUDY We have investigated the effects of an ethanol crude extract of roots of Ampelozizyphus amazonicus (CEAaD), a chemically characterized saponin mixture (SAPAaD), as well as a saponin-free fraction (SAPAaD-free) obtained from CEAaD on diuresis in rats. MATERIALS AND METHODS:
Wistar rats under ad libitum water conditions or water deprivation for 12h prior to the start of the experiment were volume-expanded with 0.9% NaCl (4% body weight, by gavage) containing either CEAaD, SAPAaD, or SAPAaD-free at the doses indicated in the text. Rats were individually housed in metabolic cages, and urine volume was measured every 30 min throughout the experiment (3 h).RESULTS:
CEAaD increased urine volume in rats under conditions of both free access to water and under water deprivation. In the latter condition, CEAaD (150 mg/kg) increased the urine volume from zero to 0.9+/-0.1 ml/120 min, n=6). Similarly, the SAPAaD-free (50-200 mg/kg) mixture also increased the urine volume. In contrast, SAPAaD (12.5-1000 mg/kg) produced a significant reduction (p<0.01) in diuresis under conditions of both water deprivation and with free access to water prior to the start of the experiment.CONCLUSION:
Our data indicate that CEAaD contains compounds that cause both diuresis and antidiuresis and that the antidiuretic effect is due mainly to the presence of saponins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saponinas
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Triterpenos
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Extratos Vegetais
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Rhamnaceae
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article