FACT and Asf1 regulate nucleosome dynamics and coactivator binding at the HO promoter.
Mol Cell
; 34(4): 405-15, 2009 May 14.
Article
em En
| MEDLINE
| ID: mdl-19481521
ABSTRACT
Transcriptional activators and coactivators overcome repression by chromatin, but regulation of chromatin disassembly and coactivator binding to promoters is poorly understood. Activation of the yeast HO gene follows the sequential binding of both sequence-specific DNA-binding proteins and coactivators during the cell cycle. Here, we show that the nucleosome disassembly occurs in waves both along the length of the promoter and during the cell cycle. Different chromatin modifiers are required for chromatin disassembly at different regions of the promoter, with Swi/Snf, the FACT chromatin reorganizer, and the Asf1 histone chaperone each required for nucleosome eviction at distinct promoter regions. FACT and Asf1 both bind to upstream elements of the HO promoter well before the gene is transcribed. The Swi/Snf, SAGA, and Mediator coactivators bind first to the far upstream promoter region and subsequently to a promoter proximal region, and FACT and Asf1 are both required for this coactivator re-recruitment.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas de Grupo de Alta Mobilidade
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Desoxirribonucleases de Sítio Específico do Tipo II
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Regiões Promotoras Genéticas
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Chaperonas Moleculares
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Proteínas de Ciclo Celular
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Proteínas de Saccharomyces cerevisiae
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Fatores de Elongação da Transcrição
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article