Receptor mediation and nociceptin inhibition of bradykinin-induced plasma extravasation in the knee joint of the rat.
Inflamm Res
; 58(12): 873-80, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19544046
ABSTRACT
OBJECTIVE AND DESIGN:
The aim was to investigate the signaling mechanisms and regulation of bradykinin (BK)-induced inflammation in rat knee joint. MATERIALS ANDMETHODS:
Knee joints of anesthetized rats were perfused with BK (0.1-1.0 microM), and synovial plasma extravasation (PE) was evaluated by spectrophotometrical measurement of Evans Blue leakage. To examine the signaling pathway, B1 antagonist [des-Arg10]-HOE140 (0.1-1.0 microM) and B2 antagonist HOE140 (0.05-1.0 microM), calcitonin gene-related peptide (CGRP) antagonist CGRP8-37 (0.5-1.0 microM), prostaglandin E2 antagonist AH-6809 (0.1-1.0 microM), and histamine H1 antagonist mepyramine (0.1-1.0 microM) were used. Nociceptin (0.0001-1.0 microM) and antagonist J-113397 were tested for modulation of BK-induced PE. The analyses were compared side-by-side with 5-hydroxytryptamine-induced PE.RESULTS:
BK perfusion dose-dependently induced PE, which was blocked by HOE140, CGRP8-37, AH-6809, and mepyramine. It was also inhibited by nociceptin, which could be reversed by antagonist J-113397. In contrast, 5-hydroxytryptamine-induced PE was biphasically regulated by nociceptin and was not antagonized by CGRP8-37.CONCLUSIONS:
BK-induced PE is mediated by B2 receptors and may involve CGRP, prostaglandin, and histamine pathways. BK-induced PE is inhibited by nociceptin through the activation of ORL1 receptors. There are differences between BK- and 5-hydroxytryptamine-induced inflammation in signaling and modulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasma
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Bradicinina
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Peptídeos Opioides
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Receptor B1 da Bradicinina
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Receptor B2 da Bradicinina
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Articulação do Joelho
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article