Virtual terminator nucleotides for next-generation DNA sequencing.
Nat Methods
; 6(8): 593-5, 2009 Aug.
Article
em En
| MEDLINE
| ID: mdl-19620973
ABSTRACT
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Análise de Sequência de DNA
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Cromossomos Artificiais Bacterianos
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Nucleotídeos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article