[Construction and identification of recombinant BHV-1 expressing foot and mouth disease virus VP1 gene].
Wei Sheng Wu Xue Bao
; 49(5): 677-82, 2009 May.
Article
em Zh
| MEDLINE
| ID: mdl-19637579
ABSTRACT
OBJECTIVE:
In order to construct the recombinant bovine hepervirus-1 (BHV-1) which expressed foot and mouth disease virus (FMDV) VP1 gene, we constructed a BHV-1 gE gene transfer vector by inserting the synthetic VP1 gene of FMDV (O/China/99) under the immediate-early promoter of cytomegalovirus.METHODS:
The mixtures of parental virus (BHV-1/gE(-)/LacZ+) DNA and transfer vector was transfected into bovine turbinate cells using calcium phosphate-mediated transfection. Then the propagated viruses were harvested. The recombinant BHV-1 (designated BHV-1/gE(-)/VP1) was obtained by selection for white virus plaques.RESULTS:
PCR results showed that VP1 gene was successfully inserted into the genome of BHV-1/gE(-). The expression of VP1 in infected cells was proved by indirect immunofluorescence assay and Western blotting.CONCLUSION:
The research provided a basis for development of BHV-1 vector vaccines for FMD and other important bovine infectious diseases.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Vacinas Virais
/
Herpesvirus Bovino 1
/
Proteínas do Capsídeo
/
Febre Aftosa
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article