Effect of meso-hexestrol, a synthetic estrogen, on S-tubulin.
Chem Pharm Bull (Tokyo)
; 38(12): 3419-22, 1990 Dec.
Article
em En
| MEDLINE
| ID: mdl-1965496
ABSTRACT
We have reported that meso-hexestrol, a synthetic estrogen, inhibits microtubule assembly and induces microtubule proteins into twisted ribbon structures. On the other hand, Serrano et al. proved that S-tubulin, which lacks the C-terminal moiety of tubulin subunits, assembles into sheet structures in the absence of microtubule-associated proteins (MAPs). In the present investigation, we attempted to clarify whether meso-hexestrol could induce the ribbon structure from S-tubulin. meso-Hexestrol delayed the initiation of polymerization of S-tubulin into sheet structures in a dose-dependent manner below 50 microM. But the effect of meso-hexestrol on S-tubulin was reduced in the presence of either tau or microtubule-associated protein 2 (MAP2) in a MAPs-concentration-dependent manner. At concentrations higher than 100 microM, meso-hexestrol inhibited the polymerization of S-tubulin into sheet structures, without forming ribbon structures. The present results may indicate that moso-hexestrol interacts with S-tubulin, and its interaction is affected by MAPs.
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Base de dados:
MEDLINE
Assunto principal:
Tubulina (Proteína)
/
Hexestrol
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article