Study on the interaction between methyl violet and bovine serum albumin by spectral analyses.
Spectrochim Acta A Mol Biomol Spectrosc
; 74(2): 526-31, 2009 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-19679509
ABSTRACT
In this article the interaction between methyl violet (MV) and bovine serum albumin (BSA) was studied with spectroscopy. The results indicated that the fluorescence intensity of BSA was quenched strongly by MV through a static quenching procedure. The association constants, the number of binding sites and basic thermodynamic parameters were obtained based on fluorescence quenching data. The effect of MV on the conformation of BSA had been investigated with synchronous fluorescence spectroscopy and circular dichroism (CD) spectrum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
/
Violeta Genciana
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article