Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years.
Virol J
; 6: 138, 2009 Sep 09.
Article
em En
| MEDLINE
| ID: mdl-19740442
ABSTRACT
BACKGROUND:
Human metapneumovirus (HMPV) is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion) gene sequences collected over a 20-year period.RESULTS:
The F gene sequences fell into two major groups, each with two subgroups, which exhibited a mean of 96% identity by predicted amino acid sequences. Amino acid identity within and between subgroups was higher than nucleotide identity, suggesting structural or functional constraints on F protein diversity. There was minimal progressive drift over time, and the genetic lineages were stable over the 20-year period. Several canonical amino acid differences discriminated between major subgroups, and polymorphic variations tended to cluster in discrete regions. The estimated rate of mutation was 7.12 x 10(-4) substitutions/site/year and the estimated time to most recent common HMPV ancestor was 97 years (95% likelihood range 66-194 years). Analysis suggested that HMPV diverged from avian metapneumovirus type C (AMPV-C) 269 years ago (95% likelihood range 106-382 years).CONCLUSION:
HMPV F protein remains conserved over decades. HMPV appears to have diverged from AMPV-C fairly recently.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Proteínas Virais de Fusão
/
Evolução Molecular
/
Metapneumovirus
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article