COP1 contributes to UVB-induced signaling in human keratinocytes.
J Invest Dermatol
; 130(2): 541-5, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-19741714
ABSTRACT
UVB irradiation has been shown to trigger a broad range of changes in gene expression in human skin; however, factors governing these events are still not well understood. In this study, we show that human constitutive photomorphogenic protein-1 (huCOP1), an E3 ligase, contributes to the orchestration of UVB response of keratinocytes. Accordingly, our data show that (i) huCOP1 protein is expressed both in the nucleus and in the cytoplasm of cultured keratinocytes, (ii) UVB reduces the levels of the huCOP1 mRNA and protein, and (iii) induces changes in the subcellular localization of huCOP1. Finally, we show that gene-specific silencing of huCOP1 induces the accumulation of the tumor suppressor p53 protein, which is further increased after UVB irradiation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Regulação da Expressão Gênica
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Ubiquitina-Proteína Ligases
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article