Analysis of transcriptome changes induced by Ptr ToxA in wheat provides insights into the mechanisms of plant susceptibility.
Mol Plant
; 2(5): 1067-83, 2009 Sep.
Article
em En
| MEDLINE
| ID: mdl-19825681
ABSTRACT
To obtain greater insight into the molecular events underlying plant disease susceptibility, we studied transcriptome changes induced by a host-selective toxin of Pyrenophora tritici-repentis, Ptr ToxA (ToxA), on its host plant, wheat. Transcriptional profiling of ToxA-treated leaves of a ToxA-sensitive wheat cultivar was performed using the GeneChip Wheat Genome Array. An improved and up-to-date annotation of the wheat microarray was generated and a new tool for array data analysis (BRAT) was developed, and both are available for public use via a web-based interface. Our data indicate that massive transcriptional reprogramming occurs due to ToxA treatment, including cellular responses typically associated with defense. In addition, this study supports previous results indicating that ToxA-induced cell death is triggered by impairment of the photosynthetic machinery and accumulation of reactive oxygen species. Based on results of this study, we propose that ToxA acts as both an elicitor and a virulence factor.
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Base de dados:
MEDLINE
Assunto principal:
Triticum
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Proteínas Fúngicas
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Regulação da Expressão Gênica de Plantas
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Perfilação da Expressão Gênica
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Micotoxinas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article