Exendin-4, a GLP-1 receptor agonist, directly induces adiponectin expression through protein kinase A pathway and prevents inflammatory adipokine expression.
Biochem Biophys Res Commun
; 390(3): 613-8, 2009 Dec 18.
Article
em En
| MEDLINE
| ID: mdl-19850014
ABSTRACT
Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor (GLP-1R) agonist that has been used as a drug injected subcutaneously for treatment of type 2 diabetes. Many studies have revealed molecular targets of Ex-4, but its influence on adipokines has not been determined. Our study showed that Ex-4 induced secretion of adiponectin into the culture medium of 3T3-L1 adipocytes. This effect of Ex-4 is due to increased adiponectin mRNA level through the GLP-1R. Both forskolin and 3-isobutyl-1-methylxanthine (IBMX), which may finally elevate cyclic adenosine monophosphate (cAMP) concentration, prevented the induction of adiponectin expression by Ex-4. Moreover, H89, a protein kinase A inhibitor, blocked the effect of Ex-4 on adiponectin. On the other hand, Ex-4 decreased the mRNA levels of inflammatory adipokines. The results indicate that Ex-4 directly promotes adiponectin secretion via the protein kinase A pathway in 3T3-L1 adipocytes and may ameliorate insulin resistance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Peçonhas
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Receptores de Glucagon
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Proteínas Quinases Dependentes de AMP Cíclico
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Peptídeo 1 Semelhante ao Glucagon
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Adipocinas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article