The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis.
Dev Biol
; 337(1): 148-56, 2010 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-19852954
ABSTRACT
Hedgehog signaling is critical for metazoan development and requires cilia for pathway activity. The gene iguana was discovered in zebrafish as required for Hedgehog signaling, and encodes a novel Zn finger protein. Planarians are flatworms with robust regenerative capacities and utilize epidermal cilia for locomotion. RNA interference of Smed-iguana in the planarian Schmidtea mediterranea caused cilia loss and failure to regenerate new cilia, but did not cause defects similar to those observed in hedgehog(RNAi) animals. Smed-iguana gene expression was also similar in pattern to the expression of multiple other ciliogenesis genes, but was not required for expression of these ciliogenesis genes. iguana-defective zebrafish had too few motile cilia in pronephric ducts and in Kupffer's vesicle. Kupffer's vesicle promotes left-right asymmetry and iguana mutant embryos had left-right asymmetry defects. Finally, human Iguana proteins (dZIP1 and dZIP1L) localize to the basal bodies of primary cilia and, together, are required for primary cilia formation. Our results indicate that a critical and broadly conserved function for Iguana is in ciliogenesis and that this function has come to be required for Hedgehog signaling in vertebrates.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Planárias
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Transdução de Sinais
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Proteínas de Transporte
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Dedos de Zinco
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Cílios
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Proteínas Adaptadoras de Transdução de Sinal
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Proteínas Hedgehog
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article