The NF kappa B independent cis-acting sequences in HIV-1 LTR responsive to T-cell activation.
J Acquir Immune Defic Syndr (1988)
; 4(2): 173-7, 1991.
Article
em En
| MEDLINE
| ID: mdl-1987353
ABSTRACT
The rate of transcription initiation directed by the long terminal repeat (LTR) of HIV-1 increases in response to mitogenic stimuli of T cells. Here we show that the response of the HIV-1 LTR may be governed by two independent sequences located 5' to the site of transcription initiation sequences that bind either NFAT-1 or NF kappa B. The rate of LTR-directed gene expression increased in response to treatment with either a phorbol ester or tumor necrosis factor alpha if either the NFAT-1 or NF kappa B binding sites were deleted, but failed to respond to these mitogenic stimuli if both sequences were absent. The HIV-1 mutant virus containing both NF kappa B and NFAT-1 deletion was able to replicate although at a much decreased growth rate, while the deletion of NFAT-1 alone increased the viral growth rate in Jurkat cells. Neither deletion of NF kappa B nor deletion of NFAT-1 decreased activation of viral replication by phorbol ester.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Ativação Linfocitária
/
Repetição Terminal Longa de HIV
/
NF-kappa B
/
HIV-1
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article