A homogeneous HLA-B*27 genotyping assay using dried reagent mixtures.
Dis Markers
; 27(2): 85-91, 2009.
Article
em En
| MEDLINE
| ID: mdl-19893203
ABSTRACT
The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to start the homogenous amplification assay. The method was validated with 229 samples also typed with an existing DELFIA-based method and results of both assays were 100% concordant. The dried reagents were shown to be stable at least up to eight weeks at room temperature without any decline in their performance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espondilite Anquilosante
/
DNA
/
Antígeno HLA-B27
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article