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Identification of alpha-Gal and non-Gal epitopes in pig corneal endothelial cells and keratocytes by using mass spectrometry.
Kim, Yun-Gon; Oh, Joo Youn; Gil, Geun-Cheol; Kim, Mee Kum; Ko, Jung Hwa; Lee, Sukmook; Lee, Hyun Ju; Wee, Won Ryang; Kim, Byung-Gee.
Afiliação
  • Kim YG; Institute of Molecular Biology and Genetics and Institute of Bioengineering, Seoul National University, Seoul, Korea.
Curr Eye Res ; 34(10): 877-95, 2009 Oct.
Article em En | MEDLINE | ID: mdl-19895316
ABSTRACT

PURPOSE:

To investigate the expression of alpha-Gal or unidentified non-Gal antigens in pig corneal endothelial cells and keratocytes, we performed the qualitative and quantitative analysis by using mass spectrometry.

METHODS:

The N-glycans from common adult pig corneal endothelial cells and keratocytes cultured in vitro were directly analyzed by using mass spectrometric approaches. In addition, immunochemical staining was added to confirm the non-Gal antigen expression in pig corneal cells.

RESULTS:

Totally, 34 of the sialylated N-glycans from pig corneal endothelial cells and 27 from pig keratocytes were identified and observed to contain nonhuman sialic acid, NeuGc as well as NeuAc. In addition, we were able to detect 25 of alpha-galactosylated N-glycan structures (22.2% of total) from the pig corneal endothelial cells and 18 of that (17.5% of total) from the pig keratocytes by using mass spectrometric approaches. On immunofluorescent staining, the expression of sialylated glycans was also observed.

CONCLUSIONS:

As well as alpha-Gal epitopes, several promising non-Gal antigens were widely expressed on both pig corneal endothelial cells and keratocytes. The detailed structural information of the alpha-Gal and non-Gal epitopes would be a tremendous value to develop a new strategy for the successful corneal xenotransplantation in future.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Endotélio Corneano / Queratinócitos / Alfa-Galactosidase / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Epitopos Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Endotélio Corneano / Queratinócitos / Alfa-Galactosidase / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Epitopos Idioma: En Ano de publicação: 2009 Tipo de documento: Article