Quantum dots enhanced ultrasensitive detection of DNA adducts.
Anal Chem
; 81(24): 10285-9, 2009 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-19925008
ABSTRACT
Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido
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Adutos de DNA
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Pontos Quânticos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article